Role of Socs3 and DNMT-1 in asthma pathophysiology in mice

Asthma is a complex genetic disease, which arises from the interaction of multiple genes and environmental stimuli.These can be mechanically explained by the Epigenetic phenomenon, which consists of the chromatin and its modifications, as well as a covalent modification of cytosines residing at the dinucleotide sequence CG in DNA by methylation. This reaction is catalyzed by a family of DNA methyltransferase enzyme (DNMTs). DNMT1 is one of them which maintained the methylation status during replication and also critical for the development, differencian and regulation of Th1 and Th2 cells as well as regulation of other genes. In this study we discuss the epigenetic regulation of of DNMT1 and Socs3 gene and their role in asthma development.For these studies we developed asthma mouse model, and used Flow cytometer, qRT2-PCR, Methylation specific PCR, bisulfate conversion and BiQ analyzer and antisense technology.

It is great privilege to express my gratitude to my both supervisors Dr. B.D. Chattopadhyay & Dr. B.N. Paul for their Guidance encouragement, moral support & faith in my ability.I am extremely thankful to Dr. C. Kesavachandran for his valuable suggestion, moral support & constructive criticism during the period of investigation & manuscript preprat.